概述

产品描述	Rabbit Polyclonal antibody recognizes SMURF2
反应物种	Hu, Ms, Rat
应用	WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	SMURF2
抗原物种	Human
抗原	Synthetic peptide of Human SMURF2.
偶联标记	Un-conjugated
別名	SMAD-specific E3 ubiquitin-protein ligase 2; hSMURF2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; EC 6.3.2.-

应用说明

应用建议	应用 推荐稀释比 WB 1:500 - 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 64750 Human SMURF2 GeneID: 66313 Mouse SMURF2 Swiss-port # A2A5Z6 Mouse E3 ubiquitin-protein ligase SMURF2 Swiss-port # Q9HAU4 Human E3 ubiquitin-protein ligase SMURF2
基因名称	SMURF2
全名	SMAD specific E3 ubiquitin protein ligase 2
生物功能	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. [UniProt]
细胞定位	Nucleus. Cytoplasm. Cell membrane. Membrane raft. Note=Cytoplasmic in the presence of SMAD7. Colocalizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. [UniProt]
预测分子量	86 kDa
翻译后修饰	Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome. [UniProt]

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