ARG59465
anti-SMURF1 antibody
anti-SMURF1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SMURF1 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Hm |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SMURF1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 326-351 of Human SMURF1. (DHNNRTTQFTDPRLHHIMNHQCQLKE) |
| 偶联标记 | Un-conjugated |
| 別名 | hSMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; EC 6.3.2.-; E3 ubiquitin-protein ligase SMURF1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9HCE7 Human E3 ubiquitin-protein ligase SMURF1 |
|---|---|
| 基因名称 | SMURF1 |
| 全名 | SMAD specific E3 ubiquitin protein ligase 1 |
| 背景介绍 | This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010] |
| 生物功能 | E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. [UniProt] |
| 预测分子量 | 86 kDa |
| 翻译后修饰 | Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404). Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
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ARG59465 anti-SMURF1 antibody ICC/IF image
Immunofluorescence: HeLa cells were blocked with 10% goat serum and then stained with ARG59465 anti-SMURF1 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59465 anti-SMURF1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59465 anti-SMURF1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59465 anti-SMURF1 antibody WB image
Western blot: Rat brain and MCF-7 whole cell lysates stained with ARG59465 anti-SMURF1 antibody at 0.5 µg/ml dilution.
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ARG59465 anti-SMURF1 antibody FACS image
Flow Cytometry: MCF-7 cells were blocked with 10% normal goat serum and then stained with ARG59465 anti-SMURF1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59465 anti-SMURF1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59465 anti-SMURF1 antibody at 1 µg/ml dilution, overnight at 4°C.
