ARG59535
anti-SMPD2 / nSMase antibody
anti-SMPD2 / nSMase antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SMPD2 / nSMase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SMPD2 / nSMase |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 9-35 of Human SMPD2 / nSMase. |
| 偶联标记 | Un-conjugated |
| 別名 | ISC1; EC 3.1.4.12; NSMASE1; Lyso-platelet-activating factor-phospholipase C; nSMase; Lyso-PAF-PLC; Sphingomyelin phosphodiesterase 2; N-SMase; NSMASE; Neutral sphingomyelinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SMPD2 |
| 全名 | sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) |
| 背景介绍 | This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009] |
| 生物功能 | Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. [UniProt] |
| 细胞定位 | Membrane; Multi-pass membrane protein. [UniProt] |
| 预测分子量 | 48 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG59535 anti-SMPD2 / nSMase antibody WB image
Western blot: 20 µg of K562 cell lysate stained with ARG59535 anti-SMPD2 / nSMase antibody at 1:1000 dilution.
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ARG59535 anti-SMPD2 / nSMase antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG59535 anti-SMPD2 / nSMase antibody (bottom histogram) or without primary antibody as control (top histogram), followed by FITC-labelled secondary antibody.
