ARG45412

anti-SMOX antibody

anti-SMOX antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes SMOX
反应物种 Hu, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SMOX
抗原物种 Human
抗原 Recombinant protein containing to human SMOX.
偶联标记 Un-conjugated
別名 SMOX; Spermine Oxidase; PAOh1; SMO; C20orf16; PAO; Polyamine Oxidase 1; DJ779E11.1; FLJ20746; MGC1010; PAO-1; Putative Cyclin G1 Interacting Protein; Chromosome 20 Open Reading Frame 16; Flavin-Containing Spermine Oxidase; Flavin Containing Amine Oxidase; EC 1.5.3.16; PAO1; PAOH

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 69 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 54498 Human SMOX

Swiss-port # Q9NWM0 Human Spermine oxidase

基因名称 SMOX
全名 Spermine Oxidase
背景介绍 Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism. [provided by RefSeq, Jul 2012]
生物功能 Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N1-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal. [UniProt]
细胞定位 Cytoplasm; Nucleus. [UniProt]
预测分子量 62 kDa

检测图片 (5) Click the Picture to Zoom In

  • ARG45412 anti-SMOX antibody IHC-P image

    Immunohistochemistry: Human endometrioid adenocarcinoma stained with ARG45412 anti-SMOX antibody at 2 μg/ml dilution.

  • ARG45412 anti-SMOX antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45412 anti-SMOX antibody at 5 μg/ml dilution.

  • ARG45412 anti-SMOX antibody WB image

    Western blot: A549 and MCF-7 stained with ARG45412 anti-SMOX antibody at 0.5 μg/ml dilution.

  • ARG45412 anti-SMOX antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45412 anti-SMOX antibody at 1 µg/10^6 cells dilution.

  • ARG45412 anti-SMOX antibody WB image

    Western blot: Rat spleen stained with ARG45412 anti-SMOX antibody at 0.5 μg/ml dilution.