ARG59433
anti-SMC6L1 antibody
anti-SMC6L1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SMC6L1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SMC6L1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to D205-E443 of Human SMC6L1. |
| 偶联标记 | Un-conjugated |
| 別名 | SMC6L1; SMC-6; Structural maintenance of chromosomes protein 6; SMC protein 6; hSMC6 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q924W5 Mouse Structural maintenance of chromosomes protein 6 Swiss-port # Q96SB8 Human Structural maintenance of chromosomes protein 6 |
|---|---|
| 基因名称 | SMC6 |
| 全名 | structural maintenance of chromosomes 6 |
| 生物功能 | Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. [UniProt] |
| 细胞定位 | Nucleus. Nucleus speckle. Chromosome. Nucleus, PML body. Chromosome, telomere. [UniProt] |
| 预测分子量 | 126 kDa |
| 翻译后修饰 | Phosphorylated. Sumoylated by NSMCE2/MMS21. Ubiquitinated. (Microbial infection) SMC5-SMC6 complex is degraded by the activity of Hepatitis B X protein. [UniProt] |
检测图片 (7) Click the Picture to Zoom In
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ARG59433 anti-SMC6L1 antibody ICC/IF image
Immunofluorescence: A431 cells were blocked with 10% goat serum and then stained with ARG59433 anti-SMC6L1 antibody (green) at 2 µg/ml dilution, overnight at 4°C.
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ARG59433 anti-SMC6L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59433 anti-SMC6L1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59433 anti-SMC6L1 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, HepG2, PANC-1, SK-OV-3, COLO-320, Rat testis and Mouse testis lysates stained with ARG59433 anti-SMC6L1 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59433 anti-SMC6L1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa, HepG2, PANC-1, SK-OV-3, COLO-320, Rat testis and Mouse testis lysates stained with ARG59433 anti-SMC6L1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG59433 anti-SMC6L1 antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59433 anti-SMC6L1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59433 anti-SMC6L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59433 anti-SMC6L1 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59433 anti-SMC6L1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59433 anti-SMC6L1 antibody at 1 µg/ml dilution, overnight at 4°C.
