ARG51691
anti-SMC1 phospho (Ser957) antibody
anti-SMC1 phospho (Ser957) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SMC1 phospho (Ser957) |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SMC1 |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 957 (G-S-S(p)-Q-G) derived from Human SMC1. |
| 偶联标记 | Un-conjugated |
| 別名 | CDLS2; SMC-1A; Structural maintenance of chromosomes protein 1A; SMC1alpha; SMC1; DXS423E; Sb1.8; SMC-1-alpha; SMC1L1; SB1.8; SMCB; SMC protein 1A |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14683 Human Structural maintenance of chromosomes protein 1A Swiss-port # Q9CU62 Mouse Structural maintenance of chromosomes protein 1A |
|---|---|
| 基因名称 | SMC1A |
| 全名 | structural maintenance of chromosomes 1A |
| 背景介绍 | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. |
| 生物功能 | Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 143 kDa |
| 翻译后修饰 | Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. |
检测图片 (2) Click the Picture to Zoom In
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ARG51691 anti-SMC1 phospho (Ser957) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with UV stained with ARG51691 anti-SMC1 phospho (Ser957) antibody.
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ARG51691 anti-SMC1 phospho (Ser957) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51691 anti-SMC1 phospho (Ser957) antibody (left) or the same antibody preincubated with blocking peptide (right).
