ARG66518
anti-SMARCB1 / BAF47 antibody
anti-SMARCB1 / BAF47 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes SMARCB1 / BAF47 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | SMARCB1 / BAF47 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human SMARCB1 / BAF47. |
| 偶联标记 | Un-conjugated |
| 別名 | RDT; BRG1-associated factor 47; Integrase interactor 1 protein; SWNTS1; RTPS1; PPP1R144; hSNFS; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1; MRD15; hSNF5; BAF47; INI1; SNF5; SNF5 homolog; SNF5L1; Snr1; Sfh1p |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 46 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q12824 Human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin s |
|---|---|
| 基因名称 | SMARCB1 |
| 全名 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 |
| 背景介绍 | The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 44 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG66518 anti-SMARCB1 / BAF47 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human astrocytoma (high magnification) stained with ARG66518 anti-SMARCB1 / BAF47 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
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ARG66518 anti-SMARCB1 / BAF47 antibody WB image
Western blot: 30 µg of Jurkat whole cell lysate stained with ARG66518 anti-SMARCB1 / BAF47 antibody at 1:1000 dilution.
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ARG66518 anti-SMARCB1 / BAF47 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human astrocytoma stained with ARG66518 anti-SMARCB1 / BAF47 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
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ARG66518 anti-SMARCB1 / BAF47 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain (high magnification) stained with ARG66518 anti-SMARCB1 / BAF47 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
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ARG66518 anti-SMARCB1 / BAF47 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain stained with ARG66518 anti-SMARCB1 / BAF47 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
