ARG40496

anti-SMAD5 phospho (Ser463 / 465) antibody

anti-SMAD5 phospho (Ser463 / 465) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes SMAD5 phospho (Ser463 / 465)
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 This antibody may cross-react with SMAD1 phospho (pS463/465) and SMAD9 phospho (pS465/467).
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SMAD5
抗原物种 Human
抗原 Phosphospecific peptide around Ser463/465 of Human SMAD5.
偶联标记 Un-conjugated
別名 DWFC; Smad5; JV5-1; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; hSmad5; MADH5; SMAD 5; MAD homolog 5; SMAD family member 5

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 17129 Mouse SMAD5

GeneID: 4090 Human SMAD5

GeneID: 59328 Rat SMAD5

基因名称 SMAD5
全名 SMAD family member 5
背景介绍 The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
生物功能 Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). [UniProt]
细胞定位 Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. [UniProt]
预测分子量 52 kDa
翻译后修饰 Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.

Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. [UniProt]

检测图片 (2) Click the Picture to Zoom In

  • ARG40496 anti-SMAD5 phospho (Ser463/465) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue stained with ARG40496 anti-SMAD5 phospho (Ser463/465) antibody.

  • ARG40496 anti-SMAD5 phospho (Ser463/465) antibody WB image

    Western blot: Mouse brain tissue lysate stained with ARG40496 anti-SMAD5 phospho (Ser463/465) antibody.