ARG22199
anti-SLC12A3 / NCC antibody
anti-SLC12A3 / NCC antibody for Electron microscopy,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SLC12A3 / NCC |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog |
| 应用 | EM, ICC/IF, IHC-P, WB |
| 特异性 | Detects ~160kDa. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 靶点名称 | SLC12A3 / NCC |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide around aa. 74-95 (N-terminus) of Rat NCC |
| 偶联标记 | Un-conjugated |
| 別名 | Solute carrier family 12 member 3; TSC; Na-Cl cotransporter; Thiazide-sensitive sodium-chloride cotransporter; Na-Cl symporter; NCCT; NCC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Slc12a3 |
| 全名 | solute carrier family 12 (sodium/chloride transporter), member 3 |
| 背景介绍 | This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. [UniProt] |
| 细胞定位 | Membrane |
| 预测分子量 | 113 kDa |
| 翻译后修饰 | Ubiquitinated; ubiquitination is essential for regulation of endocytosis. The BCR(KLHL3) complex was initially identified as a candidate ubiquitin ligase for SLC12A3 (PubMed:22406640). However, it was later shown that it is not the case. Phosphorylated in response to IL18. |
检测图片 (2) Click the Picture to Zoom In
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ARG22199 anti-SLC12A3 / NCC antibody IHC image
Immunohistochemistry: Rat kidney tissue stained with ARG22199 anti-SLC12A3 / NCC antibody at 1:200 dilution. DAPI merge.
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ARG22199 anti-SLC12A3 / NCC antibody WB image
Western blot: Rat tissue lysates stained with ARG22199 anti-SLC12A3 / NCC antibody at 1:1000 dilution.
文献引用
High-salt diet accelerates bone loss accompanied by activation of ion channels related to kidney and bone tissue in ovariectomized rats
WB / Mouse
