ARG65201
anti-SLC12A2 antibody
anti-SLC12A2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes SLC12A2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SLC12A2 |
| 抗原物种 | Human |
| 抗原 | C-NALQHSIRLSGVEDH |
| 偶联标记 | Un-conjugated |
| 別名 | BSC2; Basolateral Na-K-Cl symporter; Solute carrier family 12 member 2; potassium; PPP1R141; Bumetanide-sensitive sodium-; NKCC1; BSC |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| 研究领域 | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 131 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG65201 anti-SLC12A2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG65201 anti-SLC12A2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65201 anti-SLC12A2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Colon. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65201 anti-SLC12A2 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG65201 anti-SLC12A2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG65201 anti-SLC12A2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
