ARG45136

anti-SLC12A1 / NKCC2 antibody

anti-SLC12A1 / NKCC2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey

概述

产品描述 Rabbit Polyclonal antibody recognizes SLC12A1 / NKCC2
反应物种 Hu, Ms, Rat, Mk
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SLC12A1 / NKCC2
抗原物种 Human
抗原 Synthetic peptide corresponding to N-terminal region of human SLC12A1 / NKCC2.
偶联标记 Un-conjugated
別名 SLC12A1; Solute carrier family 12 member 1; BSC1; potassium; Solute carrier family 12 member 1; Kidney-specific Na-K-Cl symporter; Bumetanide-sensitive sodium-; NKCC2; CCC2; BSC; Bumetanide-Sensitive Cotransporter; Na-K-2Cl Cotransporter

应用说明

应用建议
应用 推荐稀释比
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 150-290 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20495 Mouse SLC12A1

GeneID: 6557 Human SLC12A1

Swiss-port # P55014 Mouse Solute carrier family 12 member 1

Swiss-port # Q13621 Human Solute carrier family 12 member 1

基因名称 SLC12A1
全名 Solute carrier family 12 member 1
背景介绍 This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]
生物功能 Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. [UniProt]
细胞定位 Membrane; Multi-pass membrane protein; Apical cell membrane. [UniProt]
预测分子量 121 kDa
翻译后修饰 Glycoprotein; Phosphoprotein. [UniProt]

检测图片 (7) Click the Picture to Zoom In

  • ARG45136 anti-SLC12A1 / NKCC2 antibody IHC-P image

    Immunohistochemistry: Human renal cancer tissue stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 2 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 5 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody WB image

    Western blot: Rat kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 0.5 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 2 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 5 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody WB image

    Western blot: Mouse kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 0.5 μg/ml dilution.

  • ARG45136 anti-SLC12A1 / NKCC2 antibody WB image

    Western blot: Monkey kidney stained with ARG45136 anti-SLC12A1 / NKCC2 antibody at 0.5 μg/ml dilution.