ARG59134
anti-SIX1 antibody
anti-SIX1 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SIX1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIX1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 245-282 of Human SIX1. (NYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDL) |
| 偶联标记 | Un-conjugated |
| 別名 | Sine oculis homeobox homolog 1; Homeobox protein SIX1; DFNA23; TIP39; BOS3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SIX1 |
| 全名 | SIX homeobox 1 |
| 背景介绍 | The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1. [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. [UniProt] |
| 预测分子量 | 32 kDa |
| 翻译后修饰 | Phosphorylated during interphase; becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements. Ubiquitinated by the anaphase promoting complex (APC), leading to its proteasomal degradation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59134 anti-SIX1 antibody WB image
Western blot: MCF-7 and 22RV1 cell lysates stained with ARG59134 anti-SIX1 antibody at 0.5 µg/ml dilution.
