ARG59330
anti-SIRT7 antibody
anti-SIRT7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SIRT7 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIRT7 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to R8-G350 of Human SIRT7. |
| 偶联标记 | Un-conjugated |
| 別名 | SIR2-like protein 7; SIR2L7; Regulatory protein SIR2 homolog 7; EC 3.5.1.-; NAD-dependent protein deacetylase sirtuin-7 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SIRT7 |
| 全名 | sirtuin 7 |
| 背景介绍 | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] |
| 生物功能 | NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis. [UniProt] |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Phosphorylated during mitosis. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59330 anti-SIRT7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse spleen stained with ARG59330 anti-SIRT7 antibody.
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ARG59330 anti-SIRT7 antibody WB image
Western blot: 50 ug of Rat spleen, 50 ug of Rat brain, 50 ug of Rat intestine, 40 ug of SMMC-7721 and 40 ug of Jurkat whole cell lysates stained with ARG59330 anti-SIRT7 antibody at 0.5 ug/ml dilution.
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ARG59330 anti-SIRT7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen stained with ARG59330 anti-SIRT7 antibody.
