ARG63423
anti-SIRT4 antibody
anti-SIRT4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes SIRT4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIRT4 |
| 抗原物种 | Human |
| 抗原 | SRCGELLPLIDPC |
| 偶联标记 | Un-conjugated |
| 別名 | SIR2L4; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; SIR2-like protein 4; EC 3.5.1.-; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; EC 2.4.2.-; Regulatory protein SIR2 homolog 4 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9Y6E7 Human NAD-dependent protein lipoamidase sirtuin-4, mitochondrial |
|---|---|
| 背景介绍 | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG63423 anti-SIRT4 antibody WB image
Western Blot: 1). Mock transfection; 2) SIRT4 (RC212226) expressing plasmid transfected HEK293 cell lysate standed with ARG63423 anti-SIRT4 antibody.
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ARG63423 anti-SIRT4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63423 anti-SIRT4 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63423 anti-SIRT4 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63423 anti-SIRT4 antibody at 2 µg/ml dilution followed by AP-staining.
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ARG63423 anti-SIRT4 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63423 anti-SIRT4 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
