ARG57749
anti-SIRT3 antibody
anti-SIRT3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SIRT3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIRT3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from Human SIRT3. |
| 偶联标记 | Un-conjugated |
| 別名 | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2L3; hSIRT3; EC 3.5.1.-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Mouse liver | ||||||||||
| 实际分子量 | ~ 44 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8R104 Mouse NAD-dependent protein deacetylase sirtuin-3 Swiss-port # Q9NTG7 Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
|---|---|
| 基因名称 | SIRT3 |
| 全名 | sirtuin 3 |
| 背景介绍 | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. [UniProt] |
| 细胞定位 | Mitochondrion matrix [UniProt] |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG57749 anti-SIRT3 antibody ICC/IF image
Immunofluorescence: MCF-7 cells stained with ARG57749 anti-SIRT3 antibody.
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ARG57749 anti-SIRT3 antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG57749 anti-SIRT3 antibody at 1:1000 dilution.
