ARG59902
anti-SIN3A antibody
anti-SIN3A antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SIN3A |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIN3A |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-120 of Human SIN3A (NP_056292.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Transcriptional corepressor Sin3a; Paired amphipathic helix protein Sin3a; Histone deacetylase complex subunit Sin3a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | 293T | ||||||||
| 实际分子量 | 165 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q60520 Mouse Paired amphipathic helix protein Sin3a Swiss-port # Q96ST3 Human Paired amphipathic helix protein Sin3a |
|---|---|
| 基因名称 | SIN3A |
| 全名 | SIN3 transcription regulator family member A |
| 背景介绍 | The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in he control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. [UniProt] |
| 细胞定位 | Nucleus. Nucleus, nucleolus. Note=Recruited to the nucleolus by SAP30L. [UniProt] |
| 预测分子量 | 145 kDa |
| 翻译后修饰 | SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59902 anti-SIN3A antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59902 anti-SIN3A antibody.
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ARG59902 anti-SIN3A antibody WB image
Western blot: 25 µg of 293T cell lysate stained with ARG59902 anti-SIN3A antibody at 1:1000 dilution.
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ARG59902 anti-SIN3A antibody IP image
Immunoprecipitation: 200 µg extracts of HeLa cells immunoprecipitated and stained with ARG59902 anti-SIN3A antibody at 1:500 dilution.
