ARG63644

anti-SIAHBP1 / FIR antibody

anti-SIAHBP1 / FIR antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

概述

产品描述 Goat Polyclonal antibody recognizes SIAHBP1 / FIR
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog
应用 IHC-P, WB
特异性 This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1)
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 SIAHBP1 / FIR
抗原物种 Human
抗原 C-YDQERFDNSDLSA
偶联标记 Un-conjugated
別名 FIR; RoBPI; Poly; SIAHBP1; Siah-binding protein 1; U; 60 kDa poly; FUSE-binding protein-interacting repressor; VRJS; Siah-BP1; Ro-binding protein 1; RoBP1; FBP-interacting repressor

应用说明

应用建议
应用 推荐稀释比
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
应用说明 WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 22827 Human PUF60

Swiss-port # Q9UHX1 Human Poly(U)-binding-splicing factor PUF60

背景介绍 The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq, Jul 2008]
研究领域 Gene Regulation antibody
预测分子量 60 kDa

检测图片 (3) Click the Picture to Zoom In

  • ARG63644 anti-SIAHBP1 / FIR antibody WB image

    Western Blot: HEK293 lysate (35 µg protein in RIPA buffer) stained with ARG63644 anti-SIAHBP1 / FIR antibody at 0.1 µg/ml dilution.

  • ARG63644 anti-SIAHBP1 / FIR antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63644 anti-SIAHBP1 / FIR antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63644 anti-SIAHBP1 / FIR antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63644 anti-SIAHBP1 / FIR antibody at 5 µg/ml dilution followed by AP-staining.