ARG63185
anti-SIAH1 antibody
anti-SIAH1 antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes SIAH1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog |
| 应用 | WB |
| 特异性 | This product is expected to recognize both reported isoforms (according to NP_003022.3; NP_001006611.1) |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIAH1 |
| 抗原物种 | Human |
| 抗原 | SRQTATALPTGTSKC |
| 偶联标记 | Un-conjugated |
| 別名 | Seven in absentia homolog 1; Siah-1; EC 6.3.2.-; SIAH1A; Siah-1a; E3 ubiquitin-protein ligase SIAH1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Neuroscience antibody |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2. |
检测图片 (2) Click the Picture to Zoom In
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ARG63185 anti-SIAH1 antibody WB image
Western Blot: Human Liver lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63185 anti-SIAH1 antibody at 1 µg/ml dilution.
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ARG63185 anti-SIAH1 antibody WB image
Western blot: 35 µg of Mouse brain (A), Rat brain (B), Mouse liver (C) and Rat liver (D) lysates (in RIPA buffer) stained with ARG63185 anti-SIAH1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
