ARG41736
anti-SHMT2 antibody
anti-SHMT2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SHMT2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SHMT2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 265-504 of Human SHMT2 (NP_005403.2). |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.1.2.1; SHMT; GLYA; Serine methylase; Glycine hydroxymethyltransferase; HEL-S-51e; Serine hydroxymethyltransferase, mitochondrial |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HeLa | ||||||
| 实际分子量 | ~ 56 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P34897 Human Serine hydroxymethyltransferase, mitochondrial |
|---|---|
| 基因名称 | SHMT2 |
| 全名 | serine hydroxymethyltransferase 2 (mitochondrial) |
| 背景介绍 | This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| 生物功能 | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. [UniProt] |
| 细胞定位 | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. Mitochondrion inner membrane. Cytoplasm. Nucleus. Note=Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985). [UniProt] |
| 预测分子量 | 56 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG41736 anti-SHMT2 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG41736 anti-SHMT2 antibody at 1:1000 dilution.
