ARG45271

anti-SGO2 antibody

anti-SGO2 antibody for Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Polyclonal antibody recognizes SGO2
反应物种 Hu
应用 FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SGO2
抗原物种 Human
抗原 Recombinant protein containing to human SGO2.
偶联标记 Un-conjugated
別名 SGO2; Shugoshin 2; TRIPIN; SGOL2; Shugoshin-Like 2; FLJ25211; Shugoshin-Like 2 (S. Pombe); Shugoshin-2; Tripin

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 180 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 151246 Human SGOL2

Swiss-port # Q562F6 Human Shugoshin-like 2

基因名称 SGO2
全名 Shugoshin 2
背景介绍 Predicted to be involved in meiotic sister chromatid cohesion. Predicted to act upstream of or within several processes, including meiotic nuclear division; meiotic sister chromatid cohesion; and positive regulation of maintenance of meiotic sister chromatid cohesion, centromeric. Located in chromosome, centromeric region and nuclear body. Part of mitotic cohesin complex. [provided by Alliance of Genome Resources, Nov 2024]
生物功能 Cooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation . [UniProt]
细胞定位 Cellular component; Centromere; Chromosome; Kinetochore; Nucleus. [UniProt]
预测分子量 145 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (3) Click the Picture to Zoom In

  • ARG45271 anti-SGO2 antibody ICC/IF image

    Immunofluorescence: U20S stained with ARG45271 anti-SGO2 antibody at 5 ug/ml dilution.

  • ARG45271 anti-SGO2 antibody WB image

    Western blot: A549, PC-3 and MOLT-4 stained with ARG45271 anti-SGO2 antibody at 0.5 μg/ml dilution.

  • ARG45271 anti-SGO2 antibody FACS image

    Flow Cytometry: CACO-2 stained with ARG45271 anti-SGO2 antibody at 1 µg/10^6 cells dilution.