ARG42900
anti-SFRS3 / SRSF3 antibody
anti-SFRS3 / SRSF3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SFRS3 / SRSF3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SFRS3 / SRSF3 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-90 of Human SFRS3 / SRSF3 (NP_003008.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Splicing factor, arginine/serine-rich 3; Serine/arginine-rich splicing factor 3; SFRS3; Pre-mRNA-splicing factor SRP20; SRp20 |
应用说明
| 应用建议 |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | U-87MG | ||||||||
| 实际分子量 | ~ 22 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P84103 Human Serine/arginine-rich splicing factor 3 Swiss-port # P84104 Mouse Serine/arginine-rich splicing factor 3 |
|---|---|
| 基因名称 | SRSF3 |
| 全名 | serine/arginine-rich splicing factor 3 |
| 背景介绍 | The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010] |
| 生物功能 | Splicing factor that specifically promotes exon-inclusion during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). Also functions as export adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). RNA-binding is semi-sequence specific (PubMed:17036044). [UniProt] |
| 细胞定位 | Nucleus. Nucleus speckle. Cytoplasm. Note=Recruited to nuclear speckles following interaction with YTHDC1. [UniProt] |
| 预测分子量 | 19 kDa |
| 翻译后修饰 | Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively phosphorylated on serine residues in the RS domain. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
-
ARG42900 anti-SFRS3 / SRSF3 antibody ICC/IF image
Immunofluorescence: NIH/3T3 cells stained with ARG42900 anti-SFRS3 / SRSF3 antibody at 1:100 dilution.
-
ARG42900 anti-SFRS3 / SRSF3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue stained with ARG42900 anti-SFRS3 / SRSF3 antibody at 1:100 dilution.
-
ARG42900 anti-SFRS3 / SRSF3 antibody WB image
Western blot: 25 µg of U-87MG cell lysate stained with ARG42900 anti-SFRS3 / SRSF3 antibody at 1:3000 dilution.
