ARG40015
anti-SF3B6 antibody
anti-SF3B6 antibody for Western blot and Human,Mouse
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SF3B6 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SF3B6 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-125 of Human SF3B6 (NP_057131.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Pre-mRNA branch site protein p14; Splicing factor 3B subunit 6; CGI-110; Spliceosome-associated protein, 14-kDa; SF3B14; SF3B14a; HSPC175; SF3b 14 kDa subunit; P14; Ht006; Splicing factor 3b, subunit 6, 14kDa; SAP14; SAP14a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse spleen and K562 | ||||
| 实际分子量 | 14 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SF3B6 |
| 全名 | splicing factor 3b, subunit 6, 14kDa |
| 背景介绍 | This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008] |
| 生物功能 | Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 15 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG40015 anti-SF3B6 antibody WB image
Western blot: Human pancreatic ductal adenocarcinoma stained with ARG40015 anti-SF3B6 antibody.
From Li J et al. Mol Med- (2021), doi: 10.1186/s10020-021-00347-7, Fig. 5. B.
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ARG40015 anti-SF3B6 antibody WB image
Western blot: 25 µg of Mouse spleen and K562 cell lysates stained with ARG40015 anti-SF3B6 antibody at 1:3000 dilution.
文献引用
The long non-coding RNA DKFZp434J0226 regulates the alternative splicing process through phosphorylation of SF3B6 in PDAC
WB / Human
