ARG45267

anti-SF3A3 antibody

anti-SF3A3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Polyclonal antibody recognizes SF3A3
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SF3A3
抗原物种 Human
抗原 Recombinant protein containing to human SF3A3.
偶联标记 Un-conjugated
別名 SF3A3; Splicing Factor 3a Subunit 3; SF3a60; SAP61; PRPF9; PRP9; Splicing Factor 3a, Subunit 3, 60kDa; Splicing Factor 3a, Subunit 3, 60kD; SAP 61; Pre-MRNA Splicing Factor SF3a (60kD); Spliceosome Associated Protein 61; Spliceosome-Associated Protein 61; Splicing Factor 3A Subunit 3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 59 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10946 Human SF3A3

GeneID: 75062 Mouse SF3A3

Swiss-port # Q12874 Human Splicing factor 3A subunit 3

Swiss-port # Q9D554 Mouse Splicing factor 3A subunit 3

基因名称 SF3A3
全名 Splicing Factor 3a Subunit 3
背景介绍 This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
生物功能 Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. [UniProt]
细胞定位 Nucleus; Spliceosome. [UniProt]
预测分子量 59 kDa
翻译后修饰 Acetylation; Phosphoprotein. [UniProt]

检测图片 (8) Click the Picture to Zoom In

  • ARG45267 anti-SF3A3 antibody IHC-P image

    Immunohistochemistry: Human larynx squamous cell carcinoma stained with ARG45267 anti-SF3A3 antibody at 2 μg/ml dilution.

  • ARG45267 anti-SF3A3 antibody ICC/IF image

    Immunofluorescence: PC-3 stained with ARG45267 anti-SF3A3 antibody at 5 ug/ml dilution.

  • ARG45267 anti-SF3A3 antibody WB image

    Western blot: Hela and A431 stained with ARG45267 anti-SF3A3 antibody at 0.5 μg/ml dilution.

  • ARG45267 anti-SF3A3 antibody FACS image

    Flow Cytometry: SiHa stained with ARG45267 anti-SF3A3 antibody at 1 µg/10^6 cells dilution.

  • ARG45267 anti-SF3A3 antibody IHC-P image

    Immunohistochemistry: Rat kidney stained with ARG45267 anti-SF3A3 antibody at 2 μg/ml dilution.

  • ARG45267 anti-SF3A3 antibody WB image

    Western blot: Rat liver and C6 stained with ARG45267 anti-SF3A3 antibody at 0.5 μg/ml dilution.

  • ARG45267 anti-SF3A3 antibody IHC-P image

    Immunohistochemistry: Mouse kidney stained with ARG45267 anti-SF3A3 antibody at 2 μg/ml dilution.

  • ARG45267 anti-SF3A3 antibody WB image

    Western blot: Mouse liver and NIH/3T3 stained with ARG45267 anti-SF3A3 antibody at 0.5 μg/ml dilution.