ARG45448
anti-SF1 antibody
anti-SF1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human SF1. |
| 偶联标记 | Un-conjugated |
| 別名 | SF1, Splicing Factor 1, ZFM1, Zinc Finger Protein 162, ZCCHC25, ZNF162, Mammalian Branch Point-Binding Protein, Zinc Finger Gene In MEN1 Locus, Transcription Factor ZFM1, MBBP, BBP, D11S636 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 68 - 80 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SF1 |
| 全名 | Splicing Factor 1 |
| 背景介绍 | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| 生物功能 | Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. [UniProt] |
| 细胞定位 | Nucleus; Spliceosome. [UniProt] |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. [UniProt]. [UniProt] |
检测图片 (8) Click the Picture to Zoom In
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Human placenta stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45448 anti-SF1 antibody at 2 μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: SK-OV-3 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
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ARG45448 anti-SF1 antibody FACS image
Flow Cytometry: A431 stained with ARG45448 anti-SF1 antibody at 1 µg/10^6 cells dilution.
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Rat lung stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: PC-12 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
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ARG45448 anti-SF1 antibody IHC-P image
Immunohistochemistry: Mouse lung stained with ARG45448 anti-SF1 antibody at 1μg/ml dilution.
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ARG45448 anti-SF1 antibody WB image
Western blot: NIH/3T3 stained with ARG45448 anti-SF1 antibody at 0.5 μg/ml dilution.
