ARG64747
anti-SEPT2 antibody
anti-SEPT2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes SEPT2 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SEPT2 |
| 抗原物种 | Human |
| 抗原 | SKQQPTQFINPET-C |
| 偶联标记 | Un-conjugated |
| 別名 | Pnutl3; NEDD-5; Septin-2; NEDD5; hNedd5; Neural precursor cell expressed developmentally down-regulated protein 5; DIFF6 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SEPT2 |
| 全名 | septin 2 |
| 生物功能 | Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 41 kDa |
检测图片 (5) Click the Picture to Zoom In
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ARG64747 anti-SEPT2 antibody WB image
Western blot: 35 µg of Human Testis lysate stained with ARG64747 anti-SEPT2 antibody at 0.05 µg/ml dilution.
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ARG64747 anti-SEPT2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64747 anti-SEPT2 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64747 anti-SEPT2 antibody WB image
Western blot: 35 µg of Human testis lysate (in RIPA buffer) stained with ARG64747 anti-SEPT2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
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ARG64747 anti-SEPT2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64747 anti-SEPT2 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG64747 anti-SEPT2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64747 anti-SEPT2 antibody at 2.5 µg/ml dilution followed by AP-staining.
