ARG44075
anti-SELENON antibody
anti-SELENON antibody for Flow cytometry,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal recognizes SELENON |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SELENON |
| 抗原物种 | Human |
| 抗原 | Human SELENON recombinant protein (Position: H260-P590). |
| 偶联标记 | Un-conjugated |
| 別名 | SELENON; Selenoprotein N; SELN; SEPN1; RSS; Selenoprotein N, 1; MDRS1; RSMD1; Rigid Spine Muscular Dystrophy 1; CMYP3; CFTD; SelN |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SELENON |
| 全名 | Selenoprotein N |
| 背景介绍 | This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in this gene are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. SEPN1-related myopathy consists of 4 autosomal recessive disorders, originally thought to be separate entities: rigid spine muscular dystrophy (RSMD1), the classical form of multiminicore disease, desmin related myopathy with Mallory-body like inclusions, and congenital fiber-type disproportion (CFTD). This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. Alternatively spliced transcript variants have been found for this gene. |
| 生物功能 | Plays an important role in cell protection against oxidative stress and in the regulation of redox-related calcium homeostasis. Regulates the calcium level of the ER by protecting the calcium pump ATP2A2 against the oxidoreductase ERO1A-mediated oxidative damage. Within the ER, ERO1A activity increases the concentration of H2O2, which attacks the luminal thiols in ATP2A2 and thus leads to cysteinyl sulfenic acid formation (-SOH) and SEPN1 reduces the SOH back to free thiol (-SH), thus restoring ATP2A2 activity. |
| 细胞定位 | Endoplasmic reticulum, Membrane |
| 预测分子量 | 66 kDa |
| 翻译后修饰 | Glycoprotein |
检测图片 (2) Click the Picture to Zoom In
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ARG44075 anti-SELENON antibody WB image
Western blot: A549, HepG2 and Hela stained with ARG44075 anti-SELENON antibody at 0.5 μg/mL dilution.
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ARG44075 anti-SELENON antibody FACS image
Flow Cytometry: HEL stained with ARG44075 anti-SELENON antibody at 1 µg/10^6 cells dilution.
