ARG43003
anti-SEH1L antibody
anti-SEH1L antibody for Immunoprecipitation,Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SEH1L |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SEH1L |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human SEH1L. |
| 偶联标记 | Un-conjugated |
| 別名 | Seh1; SEC13L; SEH1B; SEH1A; SEC13-like protein; Nucleoporin SEH1; Nup107-160 subcomplex subunit SEH1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | K562 | ||||||
| 实际分子量 | ~ 40 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | Batch dependent |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SEH1L |
| 全名 | SEH1-like nucleoporin |
| 背景介绍 | The protein encoded by this gene is part of a nuclear pore complex, Nup107-160. This protein contains WD repeats and shares 34% amino acid identity with yeast Seh1 and 30% identity with yeast Sec13. All constituents of the Nup107-160 complex, including this protein, specifically localize to kinetochores in mitosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for normal kinetochore microtubule attachment, mitotic progression and chromosome segregation. This subunit plays a role in recruitment of the Nup107-160 subcomplex to the kinetochore. As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex (PubMed:23723238). It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1 (PubMed:25457612, PubMed:27487210). [UniProt] |
| 细胞定位 | Chromosome, centromere, kinetochore. Nucleus, nuclear pore complex. Lysosome membrane. [UniProt] |
| 预测分子量 | 40 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG43003 anti-SEH1L antibody WB image
Western blot: K562 cell lysate stained with ARG43003 anti-SEH1L antibody at 1:1000 dilution.
