ARG59081
anti-SDHA antibody
anti-SDHA antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SDHA |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SDHA |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 385-664 of Human SDHA (NP_004159.2). |
| 偶联标记 | Un-conjugated |
| 別名 | FP; Fp; SDH1; PGL5; SDH2; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; CMD1GG; EC 1.3.5.1; SDHF |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | U-251MG | ||||||||
| 实际分子量 | 70kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SDHA |
| 全名 | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
| 背景介绍 | This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| 生物功能 | Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. [UniProt] |
| 细胞定位 | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt] |
| 预测分子量 | 73 kDa |
| 翻译后修饰 | Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation. Acetylated. Deacetylated by SIRT3. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59081 anti-SDHA antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG59081 anti-SDHA antibody.
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ARG59081 anti-SDHA antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat heart stained with ARG59081 anti-SDHA antibody at 1:100 dilution.
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ARG59081 anti-SDHA antibody WB image
Western blot: 25 µg of U-251MG cell lysate stained with ARG59081 anti-SDHA antibody at 1:1000 dilution.
