ARG40026

anti-SCYL2 antibody

anti-SCYL2 antibody for ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes SCYL2
反应物种 Hu, Ms, Rat
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SCYL2
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 1-300 of Human SCYL2 (NP_060458.3).
偶联标记 Un-conjugated
別名 SCY1-like protein 2; Coated vesicle-associated kinase of 104 kDa; CVAK104

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Rat brain and Mouse brain
实际分子量 104 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 213326 Mouse SCYL2

GeneID: 55681 Human SCYL2

Swiss-port # Q6P3W7 Human SCY1-like protein 2

Swiss-port # Q8CFE4 Mouse SCY1-like protein 2

基因名称 SCYL2
全名 SCY1-like, kinase-like 2
生物功能 Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. Probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. Has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. [UniProt]
细胞定位 Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Note=According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes. [UniProt]
预测分子量 104 kDa
翻译后修饰 According to PubMed:15809293, autophosphorylated in presence of poly-L-lysine. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG40026 anti-SCYL2 antibody WB image

    Western blot: 25 µg of Rat brain and Mouse brain lysates stained with ARG40026 anti-SCYL2 antibody at 1:3000 dilution.