ARG43633

anti-SCP3 antibody

anti-SCP3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes SCP3
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SCP3
抗原物种 Human
抗原 Recombinant protein fragment corresponding to a.a. 75-236 of Human SCP3 protein.
偶联标记 Un-conjugated
Protein Full name Synaptonemal complex protein 3
別名 Synaptonemal complex protein 3; SPGF4; COR1; SCP-3; SCP3

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 μg / 1X10^6 cells
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:250
WB1:500 - 1:2000
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 30-35 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 20962 Mouse SYCP3

GeneID: 50511 Human SYCP3

Swiss-port # P70281 Mouse Synaptonemal complex protein 3

Swiss-port # Q8IZU3 Human Synaptonemal complex protein 3

基因名称 SYCP3
全名 synaptonemal complex protein 3
背景介绍 This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2010]
生物功能 Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Required for efficient phosphorylation of HORMAD1 and HORMAD2 (By similarity). [UniProt]
细胞定位 Centromere; Chromosome; Nucleus
预测分子量 28 kDa
翻译后修饰 Phosphorylated. [UniProt]

检测图片 (6) Click the Picture to Zoom In

  • ARG43633 anti-SCP3 antibody IHC-P image

    Immunohistochemistry: Human gall bladder adenosquamous carcinoma stained with ARG43633 anti-SCP3 antibody at 2 μg/ml dilution.

  • ARG43633 anti-SCP3 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG43633 anti-SCP3 antibody at 5 μg/ml dilution.

  • ARG43633 anti-SCP3 antibody WB image

    Western blot: HepG2 stained with ARG43633 anti-SCP3 antibody at 0.5 μg/mL dilution.

  • ARG43633 anti-SCP3 antibody FACS image

    Flow Cytometry: JK stained with ARG43633 anti-SCP3 antibody at 1 μg / 10^6 cells dilution.

  • ARG43633 anti-SCP3 antibody WB image

    Western blot: Rat testis and C6 stained with ARG43633 anti-SCP3 antibody at 0.5 μg/mL dilution.

  • ARG43633 anti-SCP3 antibody WB image

    Western blot: Mouse testis stained with ARG43633 anti-SCP3 antibody at 0.5 μg/mL dilution.