ARG65526
anti-SCIMP antibody [NVL-07]
anti-SCIMP antibody [NVL-07] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [NVL-07] recognizes SCIMP |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IP, WB |
| 特异性 | The clone NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | NVL-07 |
| 同位型 | IgG2a |
| 靶点名称 | SCIMP |
| 抗原物种 | Human |
| 抗原 | Recombinant intracellular part of human SCIMP |
| 偶联标记 | Un-conjugated |
| 別名 | SLP65/SLP76, Csk-interacting membrane protein; UNQ5783; C17orf87; SLP adapter and CSK-interacting membrane protein |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Intracellular staining. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q6UWF3 Human SLP adapter and CSK-interacting membrane protein |
|---|---|
| 基因名称 | SCIMP |
| 全名 | SLP adaptor and CSK interacting membrane protein |
| 背景介绍 | SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles. |
| 生物功能 | Lipid tetraspanin-associated transmembrane adapter/mediator involved in major histocompatibility complex (MHC) class II signaling transduction. Required in generating the calcium response and enhancing ERK activity upon MHC-II stimulation. [UniProt] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Phosphorylated. Phosphorylation occurs on tyrosine residues by tyrosine-protein kinases LYN and SRC upon MHC-II stimulation. Palmitoylated. |
检测图片 (1) Click the Picture to Zoom In
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ARG65526 anti-SCIMP antibody [NVL-07] FACS image
Flow Cytometry: A population of SCIMP transfected HEK293T cells stained with ARG65526 anti-SCIMP antibody [NVL-07]. The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.
