ARG55924
anti-SCF antibody
anti-SCF antibody for ELISA,ICC/IF,Neutralizing,Western blot and Human
概述
| 产品描述 | Goat Polyclonal antibody recognizes SCF |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, ICC/IF, Neut, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SCF |
| 抗原物种 | Human |
| 抗原 | Recombinant Human SCF |
| 偶联标记 | Un-conjugated |
| 別名 | SCF; SHEP7; MGF; FPHH; sKITLG; Stem cell factor; KL-1; Kitl; Kit ligand; Mast cell growth factor; c-Kit ligand; FPH2; SF |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KITLG |
| 全名 | KIT ligand |
| 背景介绍 | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. [UniProt] |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain. |
检测图片 (4) Click the Picture to Zoom In
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ARG55924 anti-SCF antibody ICC/IF image
Immunocytochemistry: CACO-2 cells stained with ARG55924 anti-SCF antibody at 2 µg/ml dilution (overnight at 4°C).
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ARG55924 anti-SCF antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human SCF stained with ARG55924 anti-SCF antibody at non-reducing condition.
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ARG55924 anti-SCF antibody standard curve image
ARG55924 anti-SCF antibody results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG55924 anti-SCF antibody WB image
Western blot: 250 - 0.24 ng (left to right) of recombinant Human SCF stained with ARG55924 anti-SCF antibody at reducing condition.
