ARG65058
anti-SATB1 antibody
anti-SATB1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes SATB1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow |
| 应用 | IHC-P, WB |
| 特异性 | Reported variants NP_001124482.1 and NP_002962.1 represent identical protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SATB1 |
| 抗原物种 | Human |
| 抗原 | C-ESDEENRQKTRPRT |
| 偶联标记 | Un-conjugated |
| 別名 | Special AT-rich sequence-binding protein 1; DNA-binding protein SATB1 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010] |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 86 kDa |
| 翻译后修饰 | Sumoylated. Sumoylation promotes cleavage by caspases. Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation. Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms cannot dimerize and lose transcription regulation function because of impaired DNA and chromatin association. |
检测图片 (2) Click the Picture to Zoom In
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ARG65058 anti-SATB1 antibody WB image
Western Blot: MOLT4 lysate (35 µg protein in RIPA buffer) stained with ARG65058 anti-SATB1 antibody at 0.3 µg/ml dilution.
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ARG65058 anti-SATB1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Thymus. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65058 anti-SATB1 antibody at 3.8 µg/ml dilution followed by AP-staining.
