ARG59594
anti-SART1 antibody
anti-SART1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes SART1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SART1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-255 of Human SART1 (NP_005137.1). |
| 偶联标记 | Un-conjugated |
| 別名 | allergen Hom s 1; Snu66; Ara1; hSnu66; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1; SNU66 homolog; Squamous cell carcinoma antigen recognized by T-cells 1; HOMS1; hSART-1; SART1259; SNRNP110; SART-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Rat brain, Mouse brain and HeLa | ||||||||
| 实际分子量 | 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SART1 |
| 全名 | squamous cell carcinoma antigen recognized by T cells 1 |
| 背景介绍 | This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. [UniProt] |
| 细胞定位 | Nucleus. Note=Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. [UniProt] |
| 预测分子量 | 90 kDa |
| 翻译后修饰 | Sumoylated with SUMO2. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG59594 anti-SART1 antibody WB image
Western blot: 25 µg of Rat brain, Mouse brain and HeLa cell lysates stained with ARG59594 anti-SART1 antibody at 1:1000 dilution.
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ARG59594 anti-SART1 antibody IP image
Immunoprecipitation: 150 µg extracts of Jurkat cells immunoprecipitated and stained with ARG59594 anti-SART1 antibody at 1:1000 dilution.
