ARG11154

anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody

anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody for Western blot,ICC/IF and Virus

概述

产品描述 Rabbit Polyclonal antibody recognizes SARS-CoV / SARS-CoV-2 Spike protein (RBD)
反应物种 Virus
应用 ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SARS-CoV / SARS-CoV-2 Spike protein (RBD)
抗原物种 Virus
抗原 Recombinant SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1).
偶联标记 Un-conjugated

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:3000 - 1:5000
WB1:3000 - 1:5000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified
缓冲液 PBS, 5 mM Sodium azide and 50% Glycerol.
抗菌剂 5 mM Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (4) Click the Picture to Zoom In

  • ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image

    Immunofluorescence: HEK293 cells transfected with pCI-Neo-Mod vector including DNA encoding the SARS-CoV-2 Spike protein ACE2 binding domain (aa. 308-541 in the Spike protein sequence in Wuhan-Hu-1). This region was used as the immunogen for development of ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody. HEK293 cells stained with ARG11154 (red) at 1:3000 dilution, and co-stained with anti-beta Tubulin antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining.

  • ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image

    Western blot: 1) Non-transfected HEK293 cells, 2) HEK293 cells transfected with pCI-Neo-Mod vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA, and 3) HEK293 cells transfected with pCI-Neo-GFP vector containing the SARS-CoV-2 Spike protein ACE2 binding domain cDNA. Cell lysates were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (green) at 1:2000 dilution. The same blot was simultaneously stained with anti-beta Tubulin antibody (red) at 1:5000 dilution.

  • ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image

    Immunofluorescence: Vero cells infected with SARS-CoV-2 virus. Cells were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody (red). DAPI (blue) for nuclear staining.

  • ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody WB image

    Western blot: Full-length recombinant SARS-CoV-2 Spike protein expressed in HEK293 cells. Loading of 1 µg (left) or 100 ng (right). The blots were stained with ARG11154 anti-SARS-CoV / SARS-CoV-2 Spike protein (RBD) antibody.