ARG43729
anti-SARS-CoV-2 ORF8 antibody
anti-SARS-CoV-2 ORF8 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Virus
概述
| 产品描述 | Rabbit Polyclonal antibody recognizesSARS-CoV-2 ORF8 |
|---|---|
| 反应物种 | Virus |
| 应用 | ELISA, IHC-P, WB |
| 特异性 | ORF8 Antibody is predicted to not cross-react with other coronavirus family members. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SARS-CoV-2 ORF8 |
| 抗原物种 | Virus |
| 抗原 | Synthetic peptide corresponding to 14 amino acids near carboxyl terminus of SARS-CoV-2 (COVID-19) ORF8 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF8 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | ORF8 protein, ns8, ORF8, Non-structural protein 8 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ORF8 |
|---|---|
| 全名 | SARS-CoV-2 ORF8 |
| 背景介绍 | Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF8 may play a role in modulating host immune response (Probable). May play a role in blocking host IL17 cytokine by its interaction with host IL17RA. |
| 产品亮点 | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
检测图片 (3) Click the Picture to Zoom In
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ARG43729 anti-SARS-CoV-2 ORF8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue (left) or health control (right) lung tissue were fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43729 anti-SARS-CoV-2 ORF8 at 0.1μg/mL dilution, overnight at 4°C. Counter stained with Hematoxylin.
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ARG43729 anti-SARS-CoV-2 ORF8 antibody WB image
Western blot: 30 ng of SARS-CoV-2 ORF8 recombinant protein stained with ARG43729 anti-SARS-CoV-2 ORF8 for 1 hour incubation at RT in 5% NFDM/TBST, at 1 µg/ml (left) or 2 µg/ml (right) dilution.
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ARG43729 anti-SARS-CoV-2 ORF8 antibody ELISA image
Direct ELISA: SARS-CoV-2 ORF8 rprotein was coated on the plate and ARG43729 anti-SARS-CoV-2 ORF8 antibody was used as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
