ARG43728
anti-SARS-CoV-2 ORF7a antibody
anti-SARS-CoV-2 ORF7a antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections and Virus
概述
| 产品描述 | Rabbit Polyclonal antibody recognizesSARS-CoV-2 ORF7a |
|---|---|
| 反应物种 | Virus |
| 应用 | ELISA, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SARS-CoV-2 ORF7a |
| 抗原物种 | Virus |
| 抗原 | Synthetic peptide corresponding to 15 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF7a protein. The immunogen is located within 40-90 amino acids of the SARS-CoV-2 (COVID-19) ORF7a protein. |
| 偶联标记 | Un-conjugated |
| 別名 | ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, ORF7a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ORF7a protein, Accessory protein 7a, Protein U122, Protein X4, ORF7a |
|---|---|
| 全名 | SARS-CoV-2 ORF7a |
| 背景介绍 | Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF7a and ORF7a. ORF7a plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. It acts by binding to BST2 thereby interfering with its glycosylation. It may suppress small interfering RNA (siRNA) and may bind to host ITGAL, thereby playing a role in attachment or modulation of leukocytes. |
| 产品亮点 | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 |
检测图片 (2) Click the Picture to Zoom In
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ARG43728 anti-SARS-CoV-2 ORF7a antibody IHC-P image
Immunohistochemistry: Paraffin-embedded COVID-19 patient lung tissue (left) or health control (right) lung tissue were fixed with formaldehyde and blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG43728 anti-SARS-CoV-2 ORF7a antibody at 1μg/mL dilution, overnight at 4°C. Counter stained with Hematoxylin.
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ARG43728 anti-SARS-CoV-2 ORF7a antibody ELISA image
Direct ELISA: SARS-CoV-2 ORF7 rprotein was coated on the plate and ARG43728 anti-SARS-CoV-2 ORF7 antibody was used as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
