anti-SAPK / JNK antibody
anti-SAPK / JNK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Immune System antibody; Signaling Transduction antibody
Rabbit Polyclonal antibody recognizes SAPK / JNK
|反应物种||Hu, Ms, Rat|
|靶点名称||SAPK / JNK|
|抗原||Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK / JNK.|
|別名||MAP kinase 9; JNK2BETA; PRKM9; EC 126.96.36.199; c-Jun N-terminal kinase 2; Stress-activated protein kinase 1a; SAPK; Stress-activated protein kinase JNK2; MAPK 9; JNK2; JNK2ALPHA; JNK2A; JNK2B; SAPK1a; JNK-55; Mitogen-activated protein kinase 9; p54a; p54aSAPK|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|纯化||Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.|
|缓冲液||PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||mitogen-activated protein kinase 9|
|背景介绍||Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.|
|生物功能||Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. [UniProt]
|产品亮点||Related Antibody Duos and Panels:
ARG30294 Phospho SAPK / JNK Antibody Duo (Total, pT183/Y185)
SAPK antibodies; SAPK Duos / Panels; Anti-Rabbit IgG secondary antibodies;
|研究领域||Cancer antibody; Immune System antibody; Signaling Transduction antibody|
|翻译后修饰||Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.|
检测图片 (4) Click the Picture to Zoom In
ARG51218 nti-SAPK / JNK antibody WB image
Western blot: Mouse Hippocampus stained with ARG51218 nti-SAPK / JNK antibody.
From Ya-Shuo Zhao et al. Oxid Med Cell Longev. (2021), doi: 10.1155/2021/8520967, Fig. 6. c.
ARG51218 anti-SAPK / JNK antibody WB image
Western blot: Rat Myocardial stained with ARG51218 anti-SAPK / JNK antibody.
From Ya-Shuo Zhao et al. Oxidative Medicine and Cellular Longevity. (2019), doi: 10.1155/2019/7415212, Fig. 4. d.
ARG51218 anti-SAPK / JNK antibody WB image
Western blot: 30 µg of HeLa cells untreated or treated with TNF-alpha at 20 ng/ml dilution (10, 20, and 30 min). The blots were stained with ARG51218 anti-SAPK / JNK antibody at 1:500 dilution.
ARG51218 anti-SAPK / JNK antibody IHC-P image
Immunohistochemistry: paraffin-embedded Human breast carcinoma tissue stained with ARG51218 anti-SAPK / JNK antibody (left) or the same antibody preincubated with blocking peptide (right).