ARG51218

anti-SAPK / JNK antibody

anti-SAPK / JNK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Immune System antibody; Signaling Transduction antibody
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publication_link 参考文献6

概述

产品描述 Rabbit Polyclonal antibody recognizes SAPK / JNK
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 The antibody reacts with JNK1/2/3
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 SAPK / JNK
抗原物种 Human
抗原 Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK / JNK.
偶联标记 Un-conjugated
別名 MAPK8; Mitogen-Activated Protein Kinase 8; SAPK1; JNK1; PRKM8; JNK; Stress-Activated Protein Kinase 1c; C-Jun N-Terminal Kinase 1; JUN N-Terminal Kinase; MAP Kinase 8; MAPK9; Mitogen-Activated Protein Kinase 9; JNK2; PRKM9; SAPK; Stress-Activated Protein Kinase JNK2; Stress-Activated Protein Kinase 1a; C-Jun N-Terminal Kinase 2; MAP Kinase 9; MAPK10; Mitogen-Activated Protein Kinase 10; JNK3; P493F12; PRKM10; Stress-Activated Protein Kinase JNK3; Stress-Activated Protein Kinase 1b; C-Jun N-Terminal Kinase 3; MAP Kinase P49 3F12; MAP Kinase 10

应用说明

应用建议
应用 推荐稀释比
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 MAPK8; MAPK9; MAPK10
全名 mitogen-activated protein kinase 8; mitogen-activated protein kinase 9; mitogen-activated protein kinase 10
背景介绍 Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
生物功能 Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. [UniProt]
产品亮点 Related Antibody Duos and Panels:
ARG30294 Phospho SAPK / JNK Antibody Duo (Total, pT183/Y185)
Related products:
SAPK antibodies; SAPK Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Cancer antibody; Immune System antibody; Signaling Transduction antibody
预测分子量 48 kDa
翻译后修饰 Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.

检测图片 (4) Click the Picture to Zoom In

  • ARG51218 nti-SAPK / JNK antibody WB image

    Western blot: Mouse Hippocampus stained with ARG51218 nti-SAPK / JNK antibody.

    From Ya-Shuo Zhao et al. Oxid Med Cell Longev. (2021), doi: 10.1155/2021/8520967, Fig. 6. c.

  • ARG51218 anti-SAPK / JNK antibody WB image

    Western blot: Rat Myocardial stained with ARG51218 anti-SAPK / JNK antibody.

    From Ya-Shuo Zhao et al. Oxidative Medicine and Cellular Longevity. (2019), doi: 10.1155/2019/7415212, Fig. 4. d.

  • ARG51218 anti-SAPK / JNK antibody WB image

    Western blot: 30 µg of HeLa cells untreated or treated with TNF-alpha at 20 ng/ml dilution (10, 20, and 30 min). The blots were stained with ARG51218 anti-SAPK / JNK antibody at 1:500 dilution.

  • ARG51218 anti-SAPK / JNK antibody IHC-P image

    Immunohistochemistry: paraffin-embedded Human breast carcinoma tissue stained with ARG51218 anti-SAPK / JNK antibody (left) or the same antibody preincubated with blocking peptide (right).

客户反馈

nuts_pic      Excellent

anti-SAPK / JNK antibody

Application:WB

Sample:HeLa untreated / treated with TNF-alpha

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

anti-SAPK / JNK antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

anti-SAPK / JNK antibody

Application:WB

Sample:HeLa treated with 5 ng/ml TNF-a for 5 min.

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Involvement of Hepcidin in Cognitive Damage Induced by Chronic Intermittent Hypoxia in Mice.

WB / Mouse

Ya-Shuo Zhao et al.
Oxid Med Cell Longev.,  (2021)

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Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment.

WB / Human

Shun-Cheng Wu et al.
Biomedicines.,  (2021)

publication_link

 

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Hydrogen protects against chronic intermittent hypoxia induced renal dysfunction by promoting autophagy and alleviating apoptosis.

WB / Rat

Guan Peng et al.
Life Sci.,  (2019)

publication_link

 

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Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats.

WB / Rat

Zhao Ya-Shuo et al.
Oxidative Medicine and Cellular Longevity.,  (2019)

publication_link

 

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Interplay between Redox Signaling, Oxidative Stress, and Unfolded Protein Response (UPR) in Pathogenesis of Human Diseases.

WB / Rat

Poplawski Tomasz et al.
Oxid Med Cell Longev.,  (2019)

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Indirubin Inhibits LPS-Induced Inflammation via TLR4 Abrogation Mediated by the NF-kB and MAPK Signaling Pathways.

WB / Mouse

Lai JL et al.
Inflammation.,  (2016)

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