ARG54072
anti-SAFB1 antibody
anti-SAFB1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human
Gene Regulation antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes SAFB-1 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG1 |
| 靶点名称 | SAFB1 |
| 抗原物种 | Human |
| 抗原 | Purified recombinant human SAFB1 protein fragments expressed in E.coli. |
| 偶联标记 | Un-conjugated |
| 別名 | HET; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein; SAFB1; SAF-B; SAF-B1; Scaffold attachment factor B1; HAP |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 130 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified |
| 缓冲液 | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.2% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SAFB |
| 全名 | scaffold attachment factor B |
| 背景介绍 | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter By similarity. Can inhibit cell proliferation. |
| 生物功能 | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation. [UniProt] |
| 细胞定位 | Nucleus |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 103 kDa |
| 翻译后修饰 | Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity. |
检测图片 (2) Click the Picture to Zoom In
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ARG54072 anti-SAFB1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG54072 anti-SAFB1 antibody at 1:200 dilution.
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ARG54072 anti-SAFB1 antibody WB image
Western blot: ZR75-1 cell lysate stained with ARG54072 anti-SAFB1 antibody at 1:4000 dilution.
