ARG52419
anti-S6 Ribosomal Protein phospho (Ser 244) antibody
anti-S6 Ribosomal Protein phospho (Ser 244) antibody for Western blot and Human,Mouse
Gene Regulation antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein phospho (Ser 244) |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Chk, Rat, Gpig, NHuPrm, Xenopus laevis, Zfsh |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | S6 Ribosomal Protein |
| 抗原物种 | Mouse |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Phosphoprotein NP33; 40S ribosomal protein S6; S6 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | Specific for the ~28k rpS6 protein phosphorylated at Ser244. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RPS6 |
| 全名 | ribosomal protein S6 |
| 背景介绍 | Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5′-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011). |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. |
检测图片 (1) Click the Picture to Zoom In
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ARG52419 anti-S6 Ribosomal Protein phospho (Ser 244) antibody WB image
Western blot: Jurkat cell lysate showing specific immunolabeling of the ~28k rpS6 phosphorylated at Ser 244 (Control) by using ARG52419 anti-S6 Ribosomal Protein phospho (Ser 244) antibody.
Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide).
