ARG51205

anti-S6 Ribosomal Protein antibody

anti-S6 Ribosomal Protein antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody
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概述

产品描述 Rabbit Polyclonal antibody recognizes S6 Ribosomal Protein
反应物种 Hu
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 S6 Ribosomal Protein
抗原物种 Human
抗原 Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.
偶联标记 Un-conjugated
別名 Phosphoprotein NP33; 40S ribosomal protein S6; S6

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:100 - 1:200
IHC-P1:50 - 1:100
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6194 Human RPS6

Swiss-port # P62753 Human 40S ribosomal protein S6

基因名称 RPS6
全名 ribosomal protein S6
背景介绍 May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
生物功能 May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. [UniProt]
研究领域 Gene Regulation antibody
预测分子量 29 kDa
翻译后修饰 Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

检测图片 (3) Click the Picture to Zoom In

  • ARG51205 anti-S6 Ribosomal Protein antibody WB image

    Western blot: 20 µg of 293T and HeLa cell lysates stained with ARG51205 anti-S6 Ribosomal Protein antibody at 1:500 dilution.

  • ARG51205 anti-S6 Ribosomal Protein antibody IHC-P image

    Immunohistochemistry: paraffin- embedded human lung carcinoma tissue stained with anti-S6 Ribosomal Protein antibody ARG51205 (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51205 anti-S6 Ribosomal Protein antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF7 cells stained with anti-S6 Ribosomal Protein antibody ARG51205.

客户反馈

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anti-S6 Ribosomal Protein antibody

Application:WB

Sample:293T and HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC