ARG52420
anti-S6 Kinase 1 phospho (Thr449) antibody
anti-S6 Kinase 1 phospho (Thr449) antibody for Western blot and Arabidopsis
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes S6 Kinase 1 phospho (Thr449) |
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| 反应物种 | Arabi |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | S6 Kinase 1 |
| 抗原物种 | Arabidopsis |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1 |
应用说明
| 应用建议 |
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| 应用说明 | Specific for the ~53k S6K1 protein phosphorylated at Thr 449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | S6K1 |
| 背景介绍 | Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011). |
| 产品亮点 | Related news: Antibodies for Arabidopsis thaliana; |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 53 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG52420 anti-S6 Kinase 1 phospho (Thr449) antibody WB image
Western blot: Arabidopsis lysate showing specific immunolabeling of the ~53k S6K1 phosphorylated at Thr449 by using ARG52420 anti-S6 Kinase 1 phospho (Thr449) antibody.
Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (P-peptide), but not by the corresponding nonphospho-peptide (NP-peptide).
