ARG66749

anti-S100 antibody [SQab20182]

anti-S100 antibody [SQab20182] for IHC-Formalin-fixed paraffin-embedded sections and Human

概述

产品描述 Recombinant Rabbit Monoclonal antibody [SQab20182] recognizes S100
反应物种 Hu
应用 IHC-P
宿主 Rabbit
克隆 Monoclonal
克隆号 SQab20182
同位型 IgG
靶点名称 S100
抗原物种 Human
抗原 Synthetic peptide within aa. 50-150 of Human S100.
偶联标记 Un-conjugated
別名 S-100 protein beta chain; NEF; S100; Protein S100-B; S100 calcium-binding protein B; S100beta; S-100 protein subunit beta; S100-B

应用说明

应用建议
应用 推荐稀释比
IHC-P1:100 - 1:200
应用说明 IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
缓冲液 PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂 0.01% Sodium azide
稳定剂 40% Glycerol and 0.05% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 6285 Human S100B

Swiss-port # P04271 Human Protein S100-B

基因名称 S100B
全名 S100 calcium binding protein B
背景介绍 The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
生物功能 Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. [UniProt]
细胞定位 Cytoplasm. Nucleus. [UniProt]
预测分子量 11 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG66749 anti-S100 antibody [SQab20182] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human melanoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66749 anti-S100 antibody [SQab20182] at 18°C - 25°C for 30 minutes.