ARG64072
anti-Robo1 / DUTT1 antibody
anti-Robo1 / DUTT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Cancer antibody; Neuroscience antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes Robo1 / DUTT1 |
|---|---|
| 反应物种 | Ms |
| 预测物种 | Dog, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Robo1 / DUTT1 |
| 抗原物种 | Mouse |
| 抗原 | C-QKARPAKKQKHQ |
| 偶联标记 | Un-conjugated |
| 別名 | Deleted in U twenty twenty; H-Robo-1; DUTT1; Roundabout homolog 1; SAX3 |
应用说明
| 应用建议 |
|
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Robo1 |
| 全名 | roundabout homolog 1 (Drosophila) |
| 背景介绍 | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). May be required for lung development. [UniProt] |
| 研究领域 | Cancer antibody; Neuroscience antibody |
| 预测分子量 | 181 kDa |
| 翻译后修饰 | Ubiquitinated. May be deubiquitinated by USP33. |
检测图片 (2) Click the Picture to Zoom In
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ARG64072 anti-Robo1 / DUTT1 antibody WB image
Western Blot: fetal Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG64072 anti-Robo1 / DUTT1 antibody at 0.05 µg/ml dilution.
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ARG64072 anti-Robo1 / DUTT1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64072 anti-Robo1 / DUTT1 antibody at 5 µg/ml dilution followed by AP-staining.
