概述

产品描述	Rabbit Polyclonal antibody recognizes Rheb
反应物种	Hu
应用	FACS, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	Rheb
抗原物种	Human
抗原	Synthetic peptide corresponding to Human Rheb protein.
偶联标记	Un-conjugated
Protein Full name	GTP-binding protein Rheb
別名	GTP-binding protein Rheb; RHEB2; Ras homolog enriched in brain

应用说明

应用建议	应用 推荐稀释比 FACS 1:50 - 1:100 ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:100 WB 1:500 - 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量	20 kDa

属性

形式	Liquid
纯化	Affinity purification with immunogen.
缓冲液	PBS and 0.02% Sodium azide.
抗菌剂	0.02% Sodium azide
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 6009 Human RHEB Swiss-port # Q15382 Human GTP-binding protein Rheb
基因名称	RHEB
全名	Ras homolog enriched in brain
背景介绍	This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
生物功能	Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity. [UniProt]
细胞定位	Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Membrane
预测分子量	20 kDa
翻译后修饰	Farnesylation is important for efficiently activating mTORC1-mediated signaling. Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.

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