ARG65989
anti-Resistin antibody [1.2_3D7-1D11]
anti-Resistin antibody [1.2_3D7-1D11] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1.2_3D7-1D11] recognizes Resistin |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1.2_3D7-1D11 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | Resistin |
| 抗原物种 | Human |
| 抗原 | E. coli derived recombinant Human Resistin. (SSKTLCSMEE AINERIQEVA GSLIFRAISS IGLECQSVTS RGDLATCPRG FAVTGCTCGS ACGSWDVRAE TTCHCQCAGM DWTGARCCRV QP) |
| 偶联标记 | Un-conjugated |
| 別名 | C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; XCP1; Resistin; Adipose tissue-specific secretory factor; FIZZ3; RETN1; ADSF; Cysteine-rich secreted protein FIZZ3; RSTN |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.2) |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RETN |
| 全名 | resistin |
| 背景介绍 | This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010] |
| 生物功能 | Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. [UniProt] |
| 产品亮点 | Related products: Resistin antibodies; Resistin ELISA Kits; Anti-Mouse IgG secondary antibodies; |
| 预测分子量 | 11 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal placenta. The recommended ARG65989 anti-Resistin antibody [1.2_3D7-1D11] concentration is 1.0 µg/ml with a two hour incubation at RT. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human Resistin stained with ARG65989 anti-Resistin antibody [1.2_3D7-1D11], under non-reducing conditions.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] standard curve image
Sandwich ELISA: ARG65989 anti-Resistin antibody [1.2_3D7-1D11] as a capture antibody at 4.0 - 8.0 µg/ml combined with ARG66074 anti-Resistin antibody (Biotin) as a detection antibody at 0.5 - 1.0 µg/ml. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG65989 anti-Resistin antibody [1.2_3D7-1D11] WB image
Western blot: 250 - 0.24 ng (left to right) of Human Resistin stained with ARG65989 anti-Resistin antibody [1.2_3D7-1D11], under reducing conditions.
