ARG57108
anti-Ras (pan) antibody [2G9]
anti-Ras (pan) antibody [2G9] for Flow cytometry,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2G9] recognizes Ras (pan) |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2G9 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Ras (pan) |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-186 of Human Ras (pan) |
| 偶联标记 | Un-conjugated |
| 別名 | GTPase NRas; N-ras; NRAS1; CMNS; Transforming protein N-Ras; NCMS; ALPS4; NS6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NRAS |
| 全名 | neuroblastoma RAS viral (v-ras) oncogene homolog |
| 背景介绍 | This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011] |
| 生物功能 | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. [UniProt] |
| 预测分子量 | 21 kDa |
| 翻译后修饰 | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
检测图片 (3) Click the Picture to Zoom In
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ARG57108 anti-Ras (pan) antibody [2G9] FACS image
Flow Cytometry: HeLa cell line stained with ARG57108 anti-Ras (pan) antibody [2G9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 35 µg of 293T cell lysates stained with ARG57108 anti-Ras (pan) antibody [2G9] at 1) 1:500, 2) 1:1000, and 3) 1:5000.
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ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 200 ng of NRAS, KRAS and HRAS recombinant proteins stained with ARG57108 anti-Ras (pan) antibody [2G9].
