ARG45298
anti-RanBP2 antibody
anti-RanBP2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Polyclonal antibody recognizes RanBP2 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RanBP2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human RanBP2. |
| 偶联标记 | Un-conjugated |
| 別名 | RANBP2; RAN Binding Protein 2; NUP358; ADANE; Acute Necrotizing Encephalopathy 1 (Autosomal Dominant); Nuclear Pore Complex Protein Nup358; E3 SUMO-Protein Ligase RanBP2; Ran-Binding Protein 2; 358 KDa Nucleoporin; Nucleoporin Nup358; Nucleoporin 358; ANE1; P270; E3 SUMO-Protein Transferase RanBP2; Transformation-Related Protein 2; EC 2.3.2.-; EC 5.2.1.8 47; RanBP2; IIAE3; TRP1; TRP2== |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | 358 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RANBP2 |
| 全名 | RAN Binding Protein 2 |
| 背景介绍 | RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene encodes a very large RAN-binding protein that immunolocalizes to the nuclear pore complex. The protein is a giant scaffold and mosaic cyclophilin-related nucleoporin implicated in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly enhances SUMO1 transfer from UBC9 to the SUMO1 target SP100. These findings place sumoylation at the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are linked events. This gene is partially duplicated in a gene cluster that lies in a hot spot for recombination on chromosome 2q. [provided by RefSeq, Jul 2008] |
| 生物功能 | E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I. [UniProt] |
| 细胞定位 | Membrane; Nuclear pore complex; Nucleus. [UniProt] |
| 预测分子量 | 358 kDa |
| 翻译后修饰 | Acetylation; Disulfide bond; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG45298 anti-RanBP2 antibody IHC-P image
Immunohistochemistry: Human breast cancer stained with ARG45298 anti-RanBP2 antibody at 2 μg/ml dilution.
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ARG45298 anti-RanBP2 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG45298 anti-RanBP2 antibody at 5 ug/ml dilution.
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ARG45298 anti-RanBP2 antibody WB image
Western blot: K562 stained with ARG45298 anti-RanBP2 antibody at 0.5 μg/ml dilution.
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ARG45298 anti-RanBP2 antibody FACS image
Flow Cytometry: MCF-7 stained with ARG45298 anti-RanBP2 antibody at 1 µg/10^6 cells dilution.
