ARG41899
anti-Rab5 antibody
anti-Rab5 antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Dog,Monkey
概述
| 产品描述 | Goat Polyclonal antibody recognizes Rab5 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, IHC-Fr, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Rab5 |
| 抗原物种 | Mouse |
| 抗原 | Purified recombinant peptides within aa. 115 to the C-terminus of Mouse Rab5a, Rab5b and Rab5c. |
| 偶联标记 | Un-conjugated |
| 別名 | RAB5A: Ras-related protein Rab-5A; RAB5 RAB5B: Ras-related protein Rab-5B RAB5C: RAB5L; L1880; RAB5CL; RABL; Ras-related protein Rab-5C |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | Spleen | ||||||||||
| 实际分子量 | ~ 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | RAB5A; RAB5B; RAB5C |
|---|---|
| 全名 | RAB5A, member RAS oncogene family RAB5B, member RAS oncogene family RAB5C, member RAS oncogene family |
| 背景介绍 | Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]). [supplied by OMIM, Nov 2010] |
| 生物功能 | RAB5A: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. [UniProt] RAB5B and RAB5C: Protein transport. Probably involved in vesicular traffic. [UniProt] |
| 细胞定位 | RAB5A: Cytosol, Endosome and Plasma membrane. [UniProt] RAB5B: Endosome and Plasma membrane. [UniProt] RAB5C: Endosome and Plasma membrane. [UniProt] |
| 预测分子量 | 24 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG41899 anti-Rab5 antibody ICC/IF image
Immunofluorescence: RPE07 cells were fixed with 4% PFA. Cells were stained with ARG41899 anti-Rab5 antibody at 1:50 dilution.
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ARG41899 anti-Rab5 antibody WB image
Western blot: 100 µg of spleen lysate stained with ARG41899 anti-Rab5 antibody at 1:1000 dilution.
