ARG57073
anti-RUVBL1 / Pontin antibody [1D6]
anti-RUVBL1 / Pontin antibody [1D6] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1D6] recognizes RUVBL1 / Pontin |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1D6 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | RUVBL1 / Pontin |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-456 of Human RUVBL1 / Pontin. |
| 偶联标记 | Un-conjugated |
| 別名 | ECP-54; Pontin 52; 49 kDa TATA box-binding protein-interacting protein; ECP54; TIP49A; NMP238; EC 3.6.4.12; RuvB-like 1; TIH1; TIP49a; TIP60-associated protein 54-alpha; NMP 238; PONTIN; INO80 complex subunit H; Nuclear matrix protein 238; RVB1; INO80H; 54 kDa erythrocyte cytosolic protein; 49 kDa TBP-interacting protein; Pontin52; TIP49; TAP54-alpha |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RUVBL1 |
| 全名 | RuvB-like AAA ATPase 1 |
| 生物功能 | Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation. May be able to bind plasminogen at cell surface and enhance plasminogen activation. [UniProt] |
| 预测分子量 | 50 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57073 anti-RUVBL1 / Pontin antibody [1D6] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG57073 anti-RUVBL1 / Pontin antibody [1D6] (green) at 1:100 dilution.
DAPI (blue) for nucleus staining.
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ARG57073 anti-RUVBL1 / Pontin antibody [1D6] WB image
Western blot: 40 µg of Raji cell lysate stained with ARG57073 anti-RUVBL1 / Pontin antibody [1D6] at 1:1000.
